Structural Studies of a Potent Insect Maturation Inhibitor Bound to the Juvenile Hormone Esterase ofManduca sexta†,‡
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چکیده
منابع مشابه
Structural studies of a potent insect maturation inhibitor bound to the juvenile hormone esterase of Manduca sexta.
Juvenile hormone (JH) is an insect hormone containing an alpha,beta-unsaturated ester consisting of a small alcohol and long, hydrophobic acid. JH degradation is required for proper insect development. One pathway of this degradation is through juvenile hormone esterase (JHE), which cleaves the JH ester bond to produce methanol and JH acid. JHE is a member of the functionally divergent alpha/be...
متن کاملMechanistic studies of the degradation of juvenile hormone esterase in Manduca sexta.
The mechanisms of degradation of juvenile hormone esterase (JHE) were investigated in larvae of the tobacco hornworm, Manduca sexta. JHE is removed from the hemolymph by the pericardial cells by receptor-mediated endocytosis and is ultimately degraded in the lysosomes. Immunoprecipitation experiments and native PAGE followed by Western blotting showed that JHE associates with a putative heat sh...
متن کاملThe role of low levels of juvenile hormone esterase in the metamorphosis of Manduca sexta
The activity of juvenile hormone esterase (JHE) in feeding fifth instar larvae of Manduca sexta increases gradually with larval weight and rises to a peak after larvae pass the critical weight when juvenile hormone secretion ceases. Starvation of larvae of Manduca sexta (L.) that had exceeded the critical weight inhibited peak levels of JHE, but did not delay entry into the wandering stage when...
متن کاملCharacterization of affinity-purified juvenile hormone esterase from the plasma of the tobacco hornworm, Manduca sexta.
Juvenile hormone (JH) esterase found primarily in the hemolymph and tissues of insects is a low abundance protein involved in the ester hydrolysis of insect juvenile hormones, JHs. The enzyme was purified from the larval plasma of wild-type Manduca sexta using an affinity column prepared by binding the ligand, 3-[(4'-mercapto)butylthio]-1,1,1-trifluoropropan-2-one (MBTFP), to epoxy-activated Se...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2006
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi0521644